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Accelerated & Innovativess

Therapeutic Antibody Discovery

WIN Your Antibody Program at the Start Line
Rabbit Monoclonal Antibody Development
Yurogen offers the development of premium rabbit monoclonal antibodies (mAb), including anti idiotypes, anti post-translational modification sites, anti small molecule compounds, and rare epitopes with greater affinity and specificity. Our SMab™ antibody development platform overcomes issues with traditional methods and utilizes cutting-edge immunological techniques along with cell sorting to screen single B lymphocytes from immunized animals. Over the years, we have successfully supplied more than 10,000 different kinds of rabbit mAbs to our domestic and international clients. These antibodies are widely used in biomedical research and development as well as in clinical practices.
Why Rabbit Monoclonal Antibodies?
Service Features
Proprietary SMab™ Platform

First commercial Single B cell based platform for rabbit mAb discovery

Unique Immunization

Expertise in DNA, VLP , stable cell line and cell membrane extract immunization

Ex vivo culture of B cells

Cultured supernatant for high throughput FACS, WB or reporter cell assay etc. to help quickly narrow down positive candidates

Professional Project Design

Tailoring the immunization, sorting and screening strategy for each project

Great Experience in Rabbit mAb Discovery

Developed nearly 10,000 different kind of rabbit mAbs

Reliable Animal Source

USDA and AAALUC certified domestic animal farm

Procedure
  • Phase I: Immunization
  • Phase II: : SMab™ platform screening
  • Phase III: Rabbit mAb expression and purification
Workflow
PhaseServiceDescriptionTimeline
Phase IImmunization

  • Standard Package: Immunize two NZW rabbits fo 6-9 weeks with 4 injections and 2 bleed collections
  • Bleed titer and measured with ELISA
  • (Optional) Additional Assays: ELISA, FACS, WB & IHC etc.
  • Client selects one (or more) rabbit for splenocytes isolation
  • Phase I report

6-9 weeks
Phase IIaSingle B cell Sorting

  • Antigen specific B cell sorting (by FACS) and culture.
  • High throughput ELISA screening for cultured cell supernatant
  • Cherry pick ELISA positive clones and freeze cell pallets in RNA shield.
  • (Optional) Additional high throughput screening with remaining cells supernatant: ELISA counter screening, FACS, mini-WB & IHC,etc.

2 weeks
Phase IIb VH/VL amplification & LEM construction

  • VH/VL gene amplification from selected positive B cells
  • Linear Expression Module (LEM) construction & transfer into HEK293T cells
  • ELISA screen for transfected HEK293T cell supernatant
  • (Optional) Additional Assays: Blocking ELISA, affinity ranking, epitope mapping, FACS, WB & IHC etc.

2 weeks *
Phase IIIExpression plasmid construction & antibody expression

  • Subclone VH/VL gene into mammalian expression vector (pcDNA3.4) from selected LEM clones
  • Small scale expression and protein A/G purification of rabbit monoclonal antibodies
  • QC for purified rabbit mAbs: SDS-PAGE and/or Mass-spec, SEC-HPLC (optional)
  • Validation of purified rabbit mAbs with ELISA, or (optional) FACS, WB & IHC etc.
    Final project report

3 weeks *
* This timeline is based on the construction of 20 LEM clones and 5 final rabbit antibodies.