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Bioanalytical Services

SPR Service
Surface Plasmon Resonance (SPR) is a physical phenomenon in which linearly polarized light, under conditions of total internal reflection, undergoes resonant energy transfer at a metal-dielectric interface. This principle enables the label-free analysis of molecular interactions. Ligand molecules are immobilized on a metallic film (typically gold), followed by the injection of analytes (e.g., proteins, DNA, or small molecules). When analytes bind to the ligand, they induce changes in the refractive index at the interface, resulting in a corresponding shift in the SPR angle. By monitoring these changes in real time, SPR provides dynamic interaction data between biomolecules. This technology is widely applied in biomolecular interaction studies and drug discovery, offering unparalleled advantages over conventional methods.

Yurogen is equipped with multiple 8-channel SPR detection systems, enabling affinity analysis services for biomolecular interactions tailored to diverse user needs. These services support early-stage drug screening, downstream production quality control, and other critical applications. Committed to excellence, Yurogen serves universities, research institutions, hospitals, and pharmaceutical companies worldwide in life sciences and healthcare research.

Antigen/Antibody
Protein/Protein
Peptide/Protein
Small Molecule/Protein

Yurogen is equipped with multiple 8-channel SPR detection systems, enabling affinity analysis services for biomolecular interactions tailored to diverse user needs. These services support early-stage drug screening, downstream production quality control, and other critical applications. Committed to excellence, Yurogen serves universities, research institutions, hospitals, and pharmaceutical companies worldwide in life sciences and healthcare research.

Service Features

    High Throughput, 8-Channel System

    Analyze up to 8 ligand–analyte pairs per run with Biacore 8K–level performance.

    Fast Turnaround

    Full reports within 3 business days.

    Crude Sample Compatibility

    Directly analyze B cell supernatants, 293F expression media, and more.

    All Done in the U.S.

    Local execution ensures faster turnaround and secure, professional support.

Service Platform

Polariton Life S-CLASS Molecular Interaction Analysis System

Biacore™ T200 SPR System

Technical Principle
Case Studies
Antibody and Conventional Protein Antigen
Antibody and AAV Protein Antigen
Antibody and Oligonucleotide
VHH and Protein
Protein Receptor and Ligand
Antibody and Small-Molecule Antigens
Affinity Analysis of Biosimilars
Using a Protein A chip, a rabbit anti-human FGL1 monoclonal antibody (developed by Yurogen) was captured. The analyte, recombinant human FGL1 protein with a His tag (ABclonal, Cat: RP01268LQ), was introduced at varying concentrations. The interaction was analyzed using the Polariton Life S-CLASS system, and the equilibrium dissociation constant (KD) was determined to be 0.11 nM.
Workflow
Service TypeService DescriptionTimeline
Affinity/Kinetics Characterization Services

  • Ligand-analyte binding affinity characterization using Protein A chips.
  • Optimization of ligand immobilization, including fixation density adjustment and chip surface coupling.
  • The mobile phase consists of the corresponding analyte molecules, with at least five different concentrations tested and fitted.
  • Comprehensive affinity (KD) and binding kinetics (Ka/Kd) data are provided and sorted according to customer requirements for multiple samples.

2 weeks
High-Throughput Affinity/Kinetics Ranking and Screening

  • High-throughput screening using Protein A chips.
  • Test 1 or 2 analyte concentrations per sample pair.
  • Affinity (KD) and binding kinetics (Ka/Kd) data are obtained and sorted according to customer requirements.
  • Specificity analysis can be provided if control analytes are available.
  • One data point is defined as a single analyte at one concentration per sample pair.
  • A minimum of 10 sample pairs will be analyzed.

3 weeks

Note:

* If a specific instrument model is needed, please indicate your preference.
* Bulk sample analysis involving the same analyte or ligand may be eligible for additional discounts; please inquire for details.