(+1)508-753-4822customer@yurogen.com
English
CUSTOM SERVICES
Inquiry
High Throughput Western Blot Service
Western blot, also known as immunoblot or western blotting, is a widely utilized analytical technique in molecular biology and immunology for detecting, visualizing, distinguishing, and quantifying specific proteins in various samples. The process involves three main steps: separating proteins by size, transferring them onto a solid support, and identifying the target protein using primary and secondary antibodies.
Conventional Western blotting relies on SDS-PAGE gel electrophoresis for protein separation, followed by membrane transfer, and detection through primary and secondary antibodies in combination with colorimetric or chemiluminescent substrates. However, this method is often time-consuming, prone to errors, low throughput, and suffers from poor reproducibility.
Yurogen addresses these challenges by offering high-throughput automated Western blot services powered by Protein Simple's Abby™ platform—the next-generation chemiluminescence protein analyzer. This innovative approach promises improved efficiency, reliability, high sensitivity, and reproducibility, along with competitive pricing, rapid turnaround times, and built-in professional support.
Conventional Western blotting relies on SDS-PAGE gel electrophoresis for protein separation, followed by membrane transfer, and detection through primary and secondary antibodies in combination with colorimetric or chemiluminescent substrates. However, this method is often time-consuming, prone to errors, low throughput, and suffers from poor reproducibility.
Yurogen addresses these challenges by offering high-throughput automated Western blot services powered by Protein Simple's Abby™ platform—the next-generation chemiluminescence protein analyzer. This innovative approach promises improved efficiency, reliability, high sensitivity, and reproducibility, along with competitive pricing, rapid turnaround times, and built-in professional support.
Service Features
High-Throughput
Processing up to 72 samples per day, allowing for exceptional productivity.
Quantitative Results
Delivering fully quantitative Western blot data for precise analysis.
Fast Turnaround
Results ready within just two business days after sample receipt.
Minimum Sample Requirement
Capillary technology offers remarkable efficiency by requiring minimal amounts of proteins and primary antibodies for analysis.
Case Studies
High-Throughput Automated Western Blot Facilitates IHC Antibody Discovery
Yurogen has successfully developed rabbit monoclonal antibodies against an important inflammasome component that work in Immunohistochemistry (IHC) for one of Yurogen’s big pharma clients, utilizing Yurogen’s SMab™ platform. As many antibodies that work in Western blot (WB) tend to work in IHC as well, high-throughput automated WB was introduced in the early stage (B cell culture supernatants) to identify potential antibody clones suitable for IHC. A total of 192 ELISA-positive B cell culture supernatants from 1920 sorted B cells were screened against inflammasome component-expressing wild-type or knockout cell lines. The best candidates were then selected for rabbit IgG gene cloning and recombinant expression. IHC staining using the recombinant antibodies on wild-type and knockout cell lines was then conducted. Many clones that worked in WB also performed well in IHC.
